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Kwandika virusi ya Dengue Nucleic Acide Ikizamini (PCR-fluorescence probe uburyo)
Intangiriro
Iki gikoresho kigenewe kwandika neza virusi ya dengue nucleic aside muri serumu yabantu cyangwa plasma.Iki gikoresho gishingiye ku gice cyihariye muri genome yose ya virusi ya dengue yo mu bwoko bwa 1 ~ 4 kugirango hategurwe primers yihariye na TaqMan fluorescent probe kuri buri bwoko, kandi tumenye vuba no kwandika virusi ya dengue binyuze muri PCR yibihe nyabyo.
Ibipimo
* Ubwoko bw'icyitegererezo: Serumu cyangwa Plasma * Ibikoresho byo gusaba: ABI 7500 Sisitemu-Igihe nyacyo PCR;Bio-rad CFX96;Roche LightCycler480;SLAN PCR Sisitemu. * Ububiko -25 ℃ kugeza 8 ℃ idafunguwe kandi urinde urumuri amezi 18Ibigize 48T / Kit Ibyingenzi DENV-Ubwoko bwa reaction ivanze, lyofilize Imiyoboro 2 Primers, probe, buffer reaction ya PCR, dNTPs, Enzyme, nibindi. DENV kugenzura neza, lyofilize 1tube Plasima ya virusi ya tandem dengue ubwoko bwa 1-4 bwo kumenya ibice Kugenzura nabi (Amazi meza) 1.5mL Amazi meza Imfashanyigisho Igice kimwe / Imikorere
• Byihuse: Igihe gito cya PCR cyo kongera ibicuruzwa mubicuruzwa bisa.• Kwiyunvikana cyane & umwihariko: Guteza imbere kwisuzumisha hakiri kare kugirango bivurwe vuba.• Ubushobozi bwuzuye bwo kurwanya kwivanga.Intambwe zo gukora
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Shigella Flexneri Nucleic Acide Yipimisha (PCR-fluorescence probe uburyo)
Intangiriro
Iki gikoresho ni lisofilique acide nucleic detection reagent, yabanje gupakirwa muri fluorescent PCR 8-strip tubes kugirango hamenyekane neza aside nucleic ya Shigella flexneri (SF) mumazi, ibiryo, inyama zinyamanswa hamwe nibidukikije, kandi birakwiriye kwisuzumisha kumufasha. cyangwa gutahura Shigella flexneri.
Ibipimo
* Ubwoko bw'icyitegererezo: amazi, ibiryo, inyama z'inyamaswa hamwe n'ibidukikije. * Ibikoresho byo gusaba: ABI 7500 Sisitemu-Igihe nyacyo PCR;Bio-rad CFX96;Roche LightCycler480;SLAN PCR Sisitemu. * Ububiko -25 ℃ kugeza 8 ℃ idafunguwe kandi urinde urumuri amezi 18.Ibigize Umuyoboro umwe kuri buri kizamini Ibyingenzi 6 × 8T SF ivangavanze (ifu ya lyofilize) Imiyoboro 48 primers, probe, buffer ya PCR, dNTPs, enzymes. SF igenzura neza (ifu ya lyofilize) 1 tube Shigella flexneri yasukuye aside nucleic Kugenzura nabi (Amazi meza) 1 tube Amazi meza IFU Igice kimwe Imfashanyigisho y'abakoresha Imikorere
• Byihuse: Igihe gito cya PCR cyo kongera ibicuruzwa mubicuruzwa bisa.• Kwiyunvikana cyane & umwihariko: Guteza imbere kwisuzumisha hakiri kare kugirango bivurwe vuba.• Ubushobozi bwuzuye bwo kurwanya kwivanga.Intambwe zo gukora
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Vibrio Parahaemolyticus Nucleic Acide Ikizamini (PCR-fluorescence probe uburyo)
Intangiriro
Iki gikoresho ni lisofilique nucleic aside igaragaramo reagent, yabanje gupakirwa mumiyoboro ya fluorescent PCR 8-strip kugirango hamenyekane neza aside aside nucleic ya Vibrio parahaemolyticus (VP) mu biribwa birimo umunyu nk'ibiryo byo mu nyanja hamwe n'ibidukikije nk'amazi yo mu nyanja, kandi birakwiriye. kwisuzumisha ryabafasha cyangwa kumenya Vibrio parahaemolyticus.
Ibipimo
* Ubwoko bw'icyitegererezo: ibiryo birimo umunyu nk'ibiryo byo mu nyanja hamwe n'ibidukikije nk'amazi yo mu nyanja. * Ibikoresho byo gusaba: ABI 7500 Sisitemu-Igihe nyacyo PCR;Bio-rad CFX96;Roche LightCycler480;SLAN PCR Sisitemu. * Ububiko -25 ℃ kugeza 8 ℃ idafunguwe kandi urinde urumuri amezi 18.Ibigize Umuyoboro umwe kuri buri kizamini Ibyingenzi 6 × 8T Uruvangitirane rwa VP (ifu ya lyofilize) Imiyoboro 48 primers, probe, buffer ya PCR, dNTPs, enzymes. VP igenzura neza (ifu ya lyofilize) 1 tube Vibrio parahaemolyticus yasukuye aside nucleic Kugenzura nabi (Amazi meza) 1 tube Amazi meza IFU Igice kimwe Imfashanyigisho y'abakoresha Imikorere
• Byihuse: Igihe gito cya PCR cyo kongera ibicuruzwa mubicuruzwa bisa.• Kwiyunvikana cyane & umwihariko: Guteza imbere kwisuzumisha hakiri kare kugirango bivurwe vuba.• Ubushobozi bwuzuye bwo kurwanya kwivanga.Intambwe zo gukora
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Salmonella Enteritidis Nucleic Acide Ikizamini (PCR-fluorescence probe uburyo)
Intangiriro
Iki gikoresho ni lisofilique nucleic aside yerekana reagent, yabanje gupakirwa mumiyoboro ya fluorescent PCR 8-strip kugirango hamenyekane neza aside aside nucleique ya Salmonella enteritidis (SalE) mumazi, ibiryo, inyama zinyamanswa hamwe nibidukikije, kandi birakwiriye kwisuzumisha ubufasha. cyangwa gutahura Salmonella enteritidis.
Ibipimo
* Ubwoko bw'icyitegererezo: amazi, ibiryo, inyama z'inyamaswa hamwe n'ibidukikije. * Ibikoresho byo gusaba: ABI 7500 Sisitemu-Igihe nyacyo PCR;Bio-rad CFX96;Roche LightCycler480;SLAN PCR Sisitemu. * Ububiko -25 ℃ kugeza 8 ℃ idafunguwe kandi urinde urumuri amezi 18.Ibigize Umuyoboro umwe kuri buri kizamini Ibyingenzi 6 × 8T Imvange ya salE ivanze (ifu ya lyofilize) Imiyoboro 48 primers, probe, buffer ya PCR, dNTPs, enzymes. SalE igenzura neza (ifu ya lyofilize) 1 tube Salmonella enteritidis yasukuye aside nucleic Kugenzura nabi (Amazi meza) 1 tube Amazi meza IFU Igice kimwe Imfashanyigisho y'abakoresha Imikorere
• Byihuse: Igihe gito cya PCR cyo kongera ibicuruzwa mubicuruzwa bisa.• Kwiyunvikana cyane & umwihariko: Guteza imbere kwisuzumisha hakiri kare kugirango bivurwe vuba.• Ubushobozi bwuzuye bwo kurwanya kwivanga.Intambwe zo gukora
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Staphylococcus Aureus Nucleic Acide Ikizamini (PCR-fluorescence probe uburyo)
Intangiriro
Iki gikoresho ni lisofilique nucleic aside yerekana reagent, yabanje gupakirwa muri fluorescent PCR 8-strip tubes kugirango hamenyekane neza aside nucleic ya Staphylococcus aureus (SA) ya nucleic aside mu biryo, inyama zinyamanswa hamwe nibidukikije, kandi irakwiriye kwisuzumisha ubufasha cyangwa gutahura Staphylococcus aureus.
Ibipimo
* Ubwoko bw'icyitegererezo: ibiryo, inyama z'inyamaswa hamwe n'ibidukikije. * Ibikoresho byo gusaba: ABI 7500 Sisitemu-Igihe nyacyo PCR;Bio-rad CFX96;Roche LightCycler480;SLAN PCR Sisitemu. * Ububiko -25 ℃ kugeza 8 ℃ idafunguwe kandi urinde urumuri amezi 18.Ibigize Umuyoboro umwe kuri buri kizamini Ibyingenzi 6 × 8T SA ivanga reaction (ifu ya lyofilize) Imiyoboro 48 primers, probe, buffer ya PCR, dNTPs, enzymes. SA igenzura neza (ifu ya lyofilize) 1 tube Staphylococcus aureus yasukuye aside nucleic Kugenzura nabi (Amazi meza) 1 tube Amazi meza IFU Igice kimwe Imfashanyigisho y'abakoresha Imikorere
• Byihuse: Igihe gito cya PCR cyo kongera ibicuruzwa mubicuruzwa bisa.• Kwiyunvikana cyane & umwihariko: Guteza imbere kwisuzumisha hakiri kare kugirango bivurwe vuba.• Ubushobozi bwuzuye bwo kurwanya kwivanga.Intambwe zo gukora
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Clostridium Difficile Nucleic Acide Ikizamini (PCR-fluorescence probe uburyo)
Intangiriro
Iki gikoresho ni lisofilique nucleic aside igaragaramo reagent, yabanje gupakirwa mumiyoboro ya fluorescent PCR 8-strip kugirango hamenyekane neza aside nucleic ya Clostridium difficile (CD) mumazi, ibiryo, inyama zinyamanswa hamwe nibidukikije, kandi birakwiriye kwisuzumisha ubufasha. cyangwa gutahura Clostridium difficile.
Ibipimo
* Ubwoko bw'icyitegererezo: amazi, ibiryo, inyama z'inyamaswa hamwe n'ibidukikije. * Ibikoresho byo gusaba: ABI 7500 Sisitemu-Igihe nyacyo PCR;Bio-rad CFX96;Roche LightCycler480;SLAN PCR Sisitemu. * Ububiko -25 ℃ kugeza 8 ℃ idafunguwe kandi urinde urumuri amezi 18.Ibigize Umuyoboro umwe kuri buri kizamini Ibyingenzi 6 × 8T Imvange ya CD ivanze (ifu ya lyofilize) Imiyoboro 48 primers, probe, buffer ya PCR, dNTPs, enzymes. CD igenzura neza (ifu ya lyofilize) 1 tube Clostridium diffile isukuye nucleic aside Kugenzura nabi (Amazi meza) 1 tube Amazi meza IFU Igice kimwe Imfashanyigisho y'abakoresha Imikorere
• Byihuse: Igihe gito cya PCR cyo kongera ibicuruzwa mubicuruzwa bisa.• Kwiyunvikana cyane & umwihariko: Guteza imbere kwisuzumisha hakiri kare kugirango bivurwe vuba.• Ubushobozi bwuzuye bwo kurwanya kwivanga.Intambwe zo gukora
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Intambwe imwe RT-PCR Master mix
Intangiriro
Intambwe imwe ya RT-PCR Master Mix ni yiteguye-gukoresha, lisofilize ya master ivanze kugirango yongere imbaraga za RT-qPCR ishobora gukoreshwa mugushakisha byihuse ADN cyangwa RNA.Kuvanga harimo gufunga kabiri-gushyushya-gutangira Super HP Taq ADN polymerase, M-MLV reverse transcriptase (RNaseH-), MgCl2 nadNTPs.Ongera usubiremo master mix wongeyeho amazi yo murwego rwa PCR hamwe nicyitegererezo cyawe, Taqman probes naprimersto ubwinshi bwa 20 µl.
Ibipimo
* Ubike kuri -25 ℃ ~ 8 ℃.Kubika byumye bifunze, bitarimo ubushuhe. * Iki gitabo cyahoze cyitwa izina-Intambwe imwe RT-qPCR Master mix (hamwe na dNTPs, lyophilized).CAT no. Ibigize Ibisobanuro Umubare Icyitonderwa KY132-01 Intambwe imwe RT-PCR Master mix (hamwe na dNTPs ， lyophilized) 48T / kit 48Tubes 8-neza neza, 0.1mL Amazi yo mu rwego rwa PCR 1.5mL / Tube 1Tube Cryotube, 2.0mL KY132-02 Intambwe imwe RT-PCR Master mix (hamwe na dNTPs ， lyophilized) 48T / kit 48Tubes 8-neza neza, 0.2mL Amazi yo mu rwego rwa PCR 1.5mL / Tube 1Tube Cryotube, 2.0mL KY132-03 Intambwe imwe RT-PCR Master mix (hamwe na dNTPs ， lyophilized) 48T / kit 2Tubes Cryotube, 2.0mL Amazi yo mu rwego rwa PCR 1.5mL / Tube 1Tube Cryotube, 2.0mL KY132-04 Intambwe imwe RT-PCR Master mix (hamwe na dNTPs ， lyophilized) 500T / kit 1Tube / Amazi yo mu rwego rwa PCR 10mL / Tube 1Tube / Imikorere
• Ukuri: Ibyago bike byo kwanduza• Ibyiyumvo bihanitse: Imikorere myiza muri templates yibanze.• Ibyoroshye: Byabanje kuvangwa kandi kubuntu gukoresha. -
Maverick qPCR MQ4164 Igikoresho kigendanwa kuri Nucleic Acide Igikoresho
“Ntoya, Igendanwa kandi Byihuse”
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Umurongo Gene MiniS Sisitemu-Igihe Cyuzuye PCR
Ubushobozi bw'icyitegererezo: 16 * 0.2ml umuyoboro umwe (umuyoboro ubonerana);0.2ml 8 strip tube (umuyoboro ubonerana)
Sisitemu yo kubyitwaramo: 5 ~ 100μL
Urwego rukora: 1 ~ 1010 Amakopi / L.
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Ibikoresho bito
Kugira ngo isoko ryagutse rishoboke, Dutanga serivisi zibyibikoresho byibanze kubikorwa bya sisitemu yo mu rwego rwo hejuru itanga uburyo bwiza bwa PCR.Twishimiye kubamenyesha sisitemu esheshatu enzyme.
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Depite Nucleic Acide Ikizamini (PCR- uburyo bwa fluorescence probe)
Intangiriro
Indwara ya Mycoplasma pneumoniae itangira buhoro, hamwe nibimenyetso nko kubabara mu muhogo, kubabara umutwe, umuriro, umunaniro, kubabara imitsi, kubura ubushake bwo kurya, isesemi no kuruka mugitangira indwara.Intangiriro yumuriro mubisanzwe iringaniye, kandi ibimenyetso byubuhumekero bigaragara nyuma yiminsi 2-3, bigaragazwa ninkorora ya paroxysmal itera cyane cyane nijoro, hamwe na spucum ya mucuc cyangwa mucopurulent, rimwe na rimwe ikagira amaraso mumacandwe, ndetse na dyspnoea no kubabara mu gatuza.Muri rusange abantu barashobora kwibasirwa na Mycoplasma pneumoniae, cyane cyane mu bana batarageza ku ishuri, abana biga mu ishuri ndetse n'ingimbi.
Iki gikoresho kigenewe kwandika byujuje ubuziranenge bwa Mycoplasma pneumoniae nucleic aside muri serumu yumuntu cyangwa plasma.Iki gikoresho gikoresha cyane p1 ikurikirana cyane muri gene ya Mycoplasma pneumoniae nkakarere kayigenewe, kandi igashushanya primers yihariye hamwe na TaqMan fluorescent probe kandi ikamenya gutahura vuba no kwandika virusi ya dengue binyuze muri fluorescent PCR.
Ibipimo
* Ubwoko bw'icyitegererezo: Serumu cyangwa Plasma. * Ibikoresho byo gusaba: ABI 7500 Sisitemu-Igihe nyacyo PCR;Bio-rad CFX96;Roche LightCycler480;SLAN PCR Sisitemu. * Ububiko -25 ℃ kugeza 8 ℃ idafunguwe kandi urinde urumuri amezi 18.Ibigize 48T / Kit Ibyingenzi MP / IC reaction ivanze, lyophilized Imiyoboro 2 Primers, probe, buffer reaction ya PCR, dNTPs, Enzyme, nibindi. Depite kugenzura neza, lyophilized 1 tube Ibice bya pseudoviral harimo intego zikurikirana hamwe nuburyo bwo kugenzura imbere Kugenzura nabi (Amazi meza) 3mL Amazi meza ADN igenzura imbere, lyofilize 1 tube Ibice bya pseudoviral harimo M13 IFU Igice kimwe Imfashanyigisho y'abakoresha Imikorere
• Byihuse: Igihe gito cya PCR cyo kongera ibicuruzwa mubicuruzwa bisa.• Kwiyunvikana cyane & umwihariko: Guteza imbere kwisuzumisha hakiri kare kugirango bivurwe vuba.• Ubushobozi bwuzuye bwo kurwanya kwivanga.• Byoroshye: Ntakindi gikoresho cyo kurwanya kwanduza gikenewe.Intambwe zo gukora
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Ibikoresho byinshi byo guhumeka virusi ya Antigen Ikizamini (Uburyo bwa Zahabu Zahabu)
Icyitegererezo 1, ibisubizo 4 byikizamini, ibisubizo muminota 15
• Ifasha kumenya ibibazo byanduye
• Kugabanya ibyago byo kwisuzumisha nabi
• Tandukanya FluA & B, ADV na RSV
