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Kwandika virusi ya Dengue Nucleic Acide Ikizamini (PCR-fluorescence probe uburyo)
Intangiriro
Iki gikoresho kigenewe kwandika neza virusi ya dengue nucleic aside muri serumu yabantu cyangwa plasma.Iki gikoresho gishingiye ku gice cyihariye muri genome yose ya virusi ya dengue yo mu bwoko bwa 1 ~ 4 kugirango hategurwe primers yihariye na TaqMan fluorescent probe kuri buri bwoko, kandi tumenye vuba no kwandika virusi ya dengue binyuze muri PCR yibihe nyabyo.
Ibipimo
* Ubwoko bw'icyitegererezo: Serumu cyangwa Plasma * Ibikoresho byo gusaba: ABI 7500 Sisitemu-Igihe nyacyo PCR;Bio-rad CFX96;Roche LightCycler480;SLAN PCR Sisitemu. * Ububiko -25 ℃ kugeza 8 ℃ idafunguwe kandi urinde urumuri amezi 18Ibigize 48T / Kit Ibyingenzi DENV-Ubwoko bwa reaction ivanze, lyofilize Imiyoboro 2 Primers, probe, buffer reaction ya PCR, dNTPs, Enzyme, nibindi. DENV kugenzura neza, lyofilize 1tube Plasima ya virusi ya tandem dengue ubwoko bwa 1-4 bwo kumenya ibice Kugenzura nabi (Amazi meza) 1.5mL Amazi meza Imfashanyigisho Igice kimwe / Imikorere
• Byihuse: Igihe gito cya PCR cyo kongera ibicuruzwa mubicuruzwa bisa.• Kwiyunvikana cyane & umwihariko: Guteza imbere kwisuzumisha hakiri kare kugirango bivurwe vuba.• Ubushobozi bwuzuye bwo kurwanya kwivanga.Intambwe zo gukora
